ZFIN Image: Leerberg et al., 2017, Fig. S4

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Figure Caption

Fig. S4

Larval germ cells do not incorporate EdU.

(A-B') Single plane confocal micrographs of whole-mount wild-type larval gonads showing EdU incorporation (red). Larvae were allowed to swim freely in 200 μM EdU + 0.1%DMSO from 10 to 11 dpf (A, A') or 12 to 13 dpf (B, B'), euthanized, fixed, and processed for detection of EdU. Many SGCs are EdU-positive at both timepoints, while germ cells are consistently EdU-negative. Germ cells are labeled with Vasa (green) and nuclei are labeled with DAPI (blue). A' and B' show the EdU channel only, in grey. A,-B' are sagittal optical sections with anterior to the left. Scale bars = 20 μm.

Acknowledgments

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