ZFIN Image: Raices et al., 2017, Fig. 2

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Figure Caption

Fig. 2

Nup210 Depletion Does Not Affect Early Muscle Development

(A) Whole-mount in situ hybridization against the muscle markers Pax3, Myf5, and MyoD were performed at different times of development in zebrafish embryos injected with control or Nup210 morpholinos.

(B) Slow muscle was stained with F59 at different times of development in control and Nup210-depleted animals and analyzed by confocal microscopy.

(C) Control or Nup210 morphants were incubated with cyclopamine during development to inhibit the Sonic Hedgehog-dependent formation of early embryonic slow muscle. Addition of new muscle fibers during muscle growth was analyzed by F59 staining at 48 hpf.

Morpholino depletions were performed with n ≥ 50 embryos. Ten to 20 embryos were examined by immunofluorescence and quantified in n ≥ 3 independent experiments. Scale bars, 50 μm. See also Figure S1.

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Acknowledgments

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Reprinted from Developmental Cell, 41, Raices, M., Bukata, L., Sakuma, S., Borlido, J., Hernandez, L.S., Hart, D.O., D'Angelo, M.A., Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex, 540-554.e7, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell