Fig. S2
Sensor plasmid at 72 hpf. At 72 hpf GFP and RFP expression is equivalent after the injection of the control reporter (A, B, C). Silencing by endogenous 5′tRF-ProCGG is still present at 72hpf (D, E, F), as RFP expression is repressed. Endogenous 5′tRF-GluCTC does not efficiently silence putative targets even at 72hpf, as no alterations in RFP expression is observed when compared to GFP expression (G, H, I). Arrows indicate cells containing both GFP-reporter and mRFP-sensor. Asterisks indicate muscle fibers that lost mRFP fluorescence. Orientation of embryos: caudal, left; ventral, up. 20× magnification.