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Fig. S3
High magnification images of vinblastine-, rhein-, embelin-, hypericin-, and obatoclax-treated zebrafish larvae.
A. High magnification images of vinblastine-treated zebrafish larvae. i:Top view of vinblastine-treated zebrafish larvae at 6dpf. ii-iv:The otolith, gall bladder and intestine of vinblastine-treated zebrafish larvae at 6dpf. Scale bar:50μm.
B. High magnification imagesof rhein-treated zebrafish larvae. i:Top view of rhein-treated zebrafish larvae at 6dpf. ii-iv:The otolith, gall bladder and intestine of rhein-treated zebrafish larvae at 6dpf. v-vi: the brightfield and fluorescent images about the rheinin the intestine. IL: intestinal lumen. Scale bar: 50μm.
C. Distribution of embelinin larval and adult swim bladders. i: brightfield image of embelin-treated zebrafish larvae with the swim bladderat6dpf. ii-iii: the ventral and lateral fluorescent views of swim bladder from embelin-treated zebrafish larvae at 6dpf. Scale bar:100μm.iv: co-staining of embelin and collagen I immunofluorescence in the adult swim bladder. Scale bar: 10μm.
D. A single confocal slice of hypericin-treated larvae from the lateral view. i-ii: Scale bar:100μm.iii: Scale bar: 20μm.
E. High magnification image of the tail of obatoclax-treated larvae from the lateral view. i: Scale bar:100μm.ii: Scale bar: 10μm.