ZFIN Image: Dash et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Knockdown of sept7b reduces ?-cell volume and inhibits ?-cell proliferation.

(A,B) Three-dimensional rendered z-stack confocal images generated from control MO-injected (A) and sept7b TBMO-injected (B) zebrafish larva at 5?dpf stained as whole mounts with antibodies against insulin. (C) Insulin-positive cell volume is significantly reduced in sept7b TBMO-injected zebrafish larvae at 5?dpf. (D,E) Three-dimensional rendered z-stack confocal images generated from control MO-injected (D) and sept7b TBMO-injected (E) zebrafish larva at 5?dpf treated with EdU (green) to visualize proliferating cells and stained with antibodies against insulin (red) to visualize ?-cells. DAPI shows the nuclei (blue). Arrowheads indicate proliferating ?-cells (positive for both EdU and insulin). (F) ?-cell proliferation is significantly reduced in sept7b knockdown larvae. Error bars represent mean?�?SEM. Scale bar (A,B) (10??m); (D,E) (10??m). ***p???0.0005.

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Acknowledgments

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