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Fig. S10

ID
ZDB-IMAGE-170907-19
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Figures for Pfefferli et al., 2017
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Figure Caption

Fig. S10

Control experiments for careg lineage tracing analysis of heart and fin regeneration.

(a) Schematic representation of the transgenic strains and the experimental design for lineage tracing of careg-expressing primordial CMs during ventricle regeneration. (b-e) Immunostaining of uninjured ventricles treated with DMSO or 4-OHT using Tropomyosin (blue). Exposure to 4-OHT for 2 days is sufficient to label primordial CMs with zsYellow, as analysed at 7 days after the treatment (-1 day). (f) Schematic representation of the transgenic strains and the experimental design for lineage tracing of careg-expressing cells during fin regeneration. (g, h) Longitudinal fin sections at 0 and 4 dpa immunostaining for mCherry (red) and Zns5 (green). Exposure to 4-OHT for 1 day results in labelling of a few osteoblasts. (i-p) Live imaging of fins at different dpa after treatment with 4-OHT reveals only a few mCherry labelled cells in the stump and the regenerate. N ≥ 4.

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