ZFIN Image: Abrial et al., 2017, Fig. 2

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Fig. 2

TGF-β Signaling Is Required during PAA Progenitor Differentiation

(A–L) Stage-specific LY-364947 exposure reveals a requirement for TGF-β signaling after PAA progenitor specification. (A) Schematic diagram showing drug treatment regimens designed to inhibit TGF-β signaling during (10–16 hr post-fertilization; hpf) and/or after (+16 hpf) progenitor specification. (B–K) Left lateral views of tie1⁺ PAA angioblast clusters in 36-hpf embryos treated during the indicated developmental windows with DMSO or 50 μM LY-364947. The angioblast clusters that did form in LY-364947-treated embryos appeared smaller and/or failed to sprout (asterisks in H, J, and K). Scale bars, 200 μm. (L) Bar graph showing the average number of tie1⁺ angioblast clusters ±SD within each experimental group (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001). n = 20–50 embryos per treatment group with a total of three biological replicates.

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Acknowledgments

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