Fig. S13
Using hs:col12a1a-p2a-Cerulean transgenic fish for inducible col12a1a overexpression
(a) cerulean mRNA is robustly induced after a single heat shock in hs:col12a1a-p2a-Cerulean transgenic larvae.
(b) col12a1a overexpression increases anti-Col XII immunoreactivity in developmental Col XII domains and in ectopic sites, including the spinal cord (arrow). Shown is a maximum intensity projection and a single optical section at the level of the spinal cord. Abbreviations: sc, spinal cord.
(a-b) Views are lateral (dorsal is up, rostral is left) or transversal (section view in a; dorsal is up). BF: brightfield. Scale bars: 100 μm.