ZFIN Image: Wehner et al., 2017, Fig. S5

Image

Figure Caption

Fig. S5

Different lesion and manipulation paradigms support that Wnt/β-catenin signaling is required for axon regeneration and functional recovery after spinal cord lesion.

(a) Pharmacological interference (IWR-1) with Wnt/β-catenin signaling inhibits axon regeneration (Fischer’s exact test: ***P<0.001) and functional recovery (two-way ANOVA: F_1,98=13.71, P<0.0005; t-test: ***P<0.001) in lesioned animals.

(b) Heat shock-induced ubiquitous overexpression of the Wnt/β-catenin pathway antagonist axin1 inhibits axon regeneration (Fischer’s exact test: ***P<0.001) and functional recovery (two-way ANOVA: F_1,161=5.319, P<0.05; t-test: ***P<0.001) in lesioned hs:Axin1 transgenic animals.

(c) Heat shock-induced ubiquitous overexpression of the Wnt/β-catenin pathway antagonist dkk1 inhibits axon regeneration (Fischer’s exact test: ***P<0.001) in lesioned hs:dkk1 transgenic animals.

(d) Pharmacological interference (IWR-1) with Wnt/β-catenin signaling starting at different time points post-lesion indicates that pathway activity is required between 0.5 dpl (12 hpl) and 1 dpl for axon regeneration (Fischer’s exact test: *P<0.05, ***P<0.001, n.s. indicates not significant).

(e) Pharmacological (IWR-1) interference with Wnt/β-catenin signaling inhibits axon regeneration (Fischer’s exact test: ***P<0.001) and functional recovery (two-way ANOVA: F_1,80=8.708, P<0.0005) in stab-lesioned animals.

(a-e) Views are lateral (dorsal is up, rostral is left. BF: brightfield. Scale bars: whole mounts, 200 μm and 100 μm. Error bars indicate s.e.m.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.