IMAGE

Fig. S12

ID: ZDB-IMAGE-170825-17
Publication: Paksa et al., 2016 - Repulsive cues combined with physical barriers and cell-cell adhesion determine progenitor cell positioning during organogenesis
All Figures: Figures for Paksa et al., 2016

Image

Figure Caption

Fig. S12

PGCs are normally specified in embryos lacking the gut tube

One color (a and c) or two color (b and d) WISH on wild-type and sox32^ta56 embryos at 24hpf (a and c respectively) or 28hpf (b and d respectively). The gut is stained using foxa1 probe (a-d). In wild-type and sox32 mutant embryos the PGCs (insets) express both the early marker nos3 and the late marker piwil1. Arrowheads indicate the position of the gut (a-b), and no foxa1 expression is detected in the mutants (c-d). Number of embryos examined in a: N=40, b: N=20, c: N=9 and d: N=5. (e-f) PGCs are labeled with vasa-gfp mRNA, showing the characteristic granule formation in PGCs (insets in e and f respectively). e: N=14 and f: N=15. Scale bar 50μm. Dorsal views are shown. Anterior is up.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.