Fig. 5
Behaviour of melanophore clusters in nacre host.
Clusters of wild-type melanophores (Tg(TDL358:GFP; sox10:mRFP)) in nacre followed through metamorphosis by (a,c,e,g,h) bright-field microscopy and (b,d,f) confocal imaging. (a?h): Dorsal and ventral streaks of melanophores are visible (red and pink arrows). These melanophores gradually disappear. (e?g) On 13 and 27?dpf confocal microscopy shows a DRG labelled within the melanophore cluster. (b,d) Sox10:mRFP labels clusters of xanthophores (yellow arrows), (d,f) TDL358:GFP labels iridophores (white arrows). (e?g) The melanophore cluster extends over about six segments. (h) At 83?dpf these melanophores disappear completely leaving a ghost dark stripe pattern surrounded by light stripes. Green plus red: iridophores, red only: xanthophores, red plus black: melanophores. Scale bars, a?h: 100??m.