PUBLICATION

Homotypic cell competition regulates proliferation and tiling of zebrafish pigment cells during colour pattern formation

Authors
Walderich, B., Singh, A.P., Mahalwar, P., Nüsslein-Volhard, C.
ID
ZDB-PUB-160428-9
Date
2016
Source
Nature communications   7: 11462 (Journal)
Registered Authors
Nüsslein-Volhard, Christiane, Walderich, Brigitte
Keywords
Cell proliferation, Cell signalling, Pattern formation
MeSH Terms
  • Skin/cytology
  • Skin/embryology
  • Skin/growth & development
  • Zebrafish
  • Body Patterning*
  • Animals
  • Cell Communication
  • Microscopy, Confocal
  • Color*
  • Skin Pigmentation*
  • Melanophores/cytology
  • Melanophores/metabolism
  • Blastomeres/cytology
  • Blastomeres/metabolism
  • Cell Proliferation*
  • Chromatophores/cytology*
  • Chromatophores/metabolism
(all 17)
PubMed
27118125 Full text @ Nat. Commun.
Abstract
The adult striped pattern of zebrafish is composed of melanophores, iridophores and xanthophores arranged in superimposed layers in the skin. Previous studies have revealed that the assembly of pigment cells into stripes involves heterotypic interactions between all three chromatophore types. Here we investigate the role of homotypic interactions between cells of the same chromatophore type. Introduction of labelled progenitors into mutants lacking the corresponding cell type allowed us to define the impact of competitive interactions via long-term in vivo imaging. In the absence of endogenous cells, transplanted iridophores and xanthophores show an increased rate of proliferation and spread as a coherent net into vacant space. By contrast, melanophores have a limited capacity to spread in the skin even in the absence of competing endogenous cells. Our study reveals a key role for homotypic competitive interactions in determining number, direction of migration and individual spacing of cells within chromatophore populations.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b2
    		Unknown
    b4
      		Insertion
      i149TgTransgenic Insertion
        i186TgTransgenic Insertion
          j9s1
            		Point Mutation
            t32238TgTransgenic Insertion
              t32239TgTransgenic Insertion
                tdl358EtTransgenic Insertion
                  tm236b
                    		Point Mutation
                    w2
                      		Point Mutation
                      1 - 10 of 11
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      mRFPEFGmRFP
                      NTREFGNTR
                      1 - 6 of 6
                      Show
                      Mapping
                      No data available
                      Your Input Welcome
                      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                      Citation
                      Walderich, B., Singh, A.P., Mahalwar, P., Nüsslein-Volhard, C. (2016) Homotypic cell competition regulates proliferation and tiling of zebrafish pigment cells during colour pattern formation. Nature communications. 7:11462.