ZFIN Image: Lister et al., 2014, Fig. 1

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Fig. 1

The microphthalmia-associated transcription factor (MITF) is oncogenic with BRAF^V600E in melanomagenesis. (a) Adult wild-type zebrafish or (b) adult mitfa^vc7 mutant zebrafish living in water at 28 °C or (c, d) <26 °C. At <26 °C some melanocytes are visible in the body (d: enlarged region, white arrows). (e) Adult transgenic line expressing human BRAF^V600E in the melanocytes. (f–i) Genetic crosses of BRAF^V600Emitf at the semirestrictive temperatures develop nevi (*) and melanoma (on the tail of the middle fish, and on the head of the bottom fish). (j) Melanoma incidence curves of BRAF^V600Ep53 and BRAF^V600Emitf (<26 °C) genetic crosses. (k) Real-time PCR (RT-PCR) analysis of the mitfa transcript in BRAF^V600Ep53 and BRAF^V600Emitf melanomas.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Invest. Dermatol.