ZFIN Image: Murphy et al., 2017, Fig. 13

Image

Figure Caption

Fig. 13

Induction of ectopic cell death is not sufficient to disrupt eng2a expression in the anterior hindbrain of wildtype embryos.

TUNEL assay and in situ hybridization to eng2a RNA in wildtype embryos in which cell death is induced by Bcl2 inhibitor HA14–1. Wildtype DMSO (A,B,C) and HA14–1 (D,E,F) treated embryos were assessed for apoptotic nuclei by TUNEL assay followed by laser scanning confocal microscopy (brightfield A,D; darkfield B,E); and for eng2a expression by in situ hybridization (C,F). Embryos exhibit normal eng2a expression in the anterior hindbrain domain (C and F), regardless of the induction of higher levels of cell death by Bcl2 inhibitor treatment compared to controls (B versus E). Refer to F and for quantitative analysis.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 146, Murphy, T., Melville, H., Fradkin, E., Bistany, G., Branigan, G., Olsen, K., Comstock, C.R., Hanby, H., Garbade, E., DiBenedetto, A.J., Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish, 10-30, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.