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Fig. 4
SINV does not induce BBB leakage. In vivo assay for BBB permeability. Uninfected controls, IHNV- and SINV-infected fliG/U:RFP larvae were injected IV at 1 and 2?dpi with 10?kDa FITC-dextran. One hour later, live confocal imaging of the brain was performed, to compare FITC intensity within the CBV and BP. (Injections at 2?dpi were performed only on uninfected controls and SINV-infected larvae, because of early death of IHNV-infected larvae.) (A,B) Representative images of IHNV-infected (A) and SINV-infected (B) larvae; single confocal plane at same depth. Scale bars: 100??m. (C) Ratios of FITC fluorescence intensities measured in BP and CBV. n=2-5 larvae per group; two separate focal planes per larva.