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Fig. 1
SINV replicates in zebrafish larvae and exhibits a broad organ tropism. (A) Scheme of a 72?hours post-fertilization (hpf) larva, showing the sites of injection: IV, intravenously in the caudal vein (CV) or the dorsal aorta (DA); IC, intracerebrally in the optic tectum; eye, in the retina. (B) Virus replication in IV-infected zebrafish larvae, assessed by titration of homogenates of whole larvae. Data represent the meansąs.e.m. of five individual larvae per time point, from three experiments pooled. (C) Survival curves of control uninfected (No V) and IV-infected zebrafish larvae (SINV). Data were pooled from five independent experiments. n=60 larvae per group. (D) Live detection of SINV-infected cells by in vivo confocal imaging, with superposition of transmitted light and GFP fluorescence (maximal projection). (D?) Uninfected control (No V), 4?dpf (1?dpi). (D?,D?) The same IV SINV-infected (SINV) larva at 1?dpi (D?) and 2?dpi (D?). H, heart (dotted red line); L, liver (dotted yellow line); Y, yolk (note that the yolk is autofluorescent but, as shown in D?, at a nearly undetectable level using these imaging conditions; the signal in D? corresponds to infection); white arrowhead, infection in the left pectoral muscle; blue arrowhead, infection in the swim bladder; orange arrowhead, infection in the brain. Scale bars: 50??m. In this and all following lateral view figures, anterior is to the left and dorsal to the top.