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Fig. 5
Changes in Granule Cell Representations Correlate with a Behavioral Change in Sensitivity to Sensory Stimuli
(A) Upper-left trace: probability of a behavioral response to visual stimuli in the ten trials immediately following injection of 10 mM bicuculline, a GABAA receptor antagonist, into the cerebellum (n = 11). The average behavior is shown with shaded SE. Note that no electrical stimulus was presented in this subset of experiments. Lower-left trace: behavioral responses in these same animals recover as bicuculline washes out. Lower-right trace: sham injections into the cerebellum failed to elicit any increase in luminance-evoked behavior. Upper right: example epifluorescence micrographs showing the extent of the bicuculline (with rhodamine) injections. The scale bar represents 100 μm. The shaded region in behavioral traces indicates the SE.
(B) Heatmaps from the same imaging plane through the granule cell layer in a 7-dpf GR152:gal4; UAS:GCaMP6s fish showing the change in pixelwise correlations to luminance (upper) and motor (lower) regressors following the addition of bicuculline. The scale bar represents 50 μm. Colored circles indicate example granule cells analyzed in subsequent panels.
(C) Heatmaps of activity from (i) two example granule cells that change their responses following the addition of bicuculline. The upper cell, gc 1, becomes less responsive to luminance and more responsive to motor activity, whereas the bottom cell, gc 2, becomes newly responsive to luminance and motor activity. Location of granule cells is shown in (B). (ii) Heatmap showing the behavioral responses over the experiment.
(D) The plotted change in regressor over trials for the two example granule cells, color coded for the two example cells as in (Ci). The gray bar indicates the presence of 30 μm bicuculline.