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Fig. S2
Symptoms of Bd infection in zebrafish larvae (Related to Figure 2).
(a) Zebrafish larvae bath water was inoculated with mTGhL (control) or high (> 200 zsp/μl) dose Bd zoospores, incubated for 72 hpi and imaged by stereomicroscopy. Cartoon depicts imaged region. Representative images with arrows highlighting 25 blisters on infected larva. (b) Zebrafish larvae bath water was inoculated with heat killed or high dose Bd zoospores, incubated for 72 hpi and imaged by stereomicroscopy. Representative images with cyan outline showing presence of fin over time. (c) Zebrafish larvae bath water was inoculated with control (open circles), low dose Bd zoospores ( < 200 zsp/μl, filled circles) high dose Bd supernatant (open squares) or high dose Bd zoospores (filled squares). Larvae were incubated for 72 hpi and dorsal fin length was measured at 24, 48 and 72 hpi. Each point represents fin length on an individual larva. Data pooled from three experiments per dose, using n = 3 – 4 per treatment. Mean ± SEM are shown. Significance testing performed using unpaired student’s t-test (two-tailed), ns p > 0.05, *** p < 0.001. (d) Bd broth culture was labelled with calcofluor white (CFW; for chitin, green) using the same protocol as in zebrafish larvae and imaged by fluorescent stereomicroscopy. Representative images showing colocalisation of CFW with Bd sporangium. Scale bar = 50 μm. (e) Enumeration of CFW-labelled punctae from larvae whose bath water was inoculated with control (open circles), low (filled circles) or high (filled squares) dose Bd zoospores and incubated for 72 hpi. Each point represents an individual larva, using n = 12 per treatment. Mean ± SEM are shown. Showing replicate experiments used in Fig. 2d. Significance testing performed using Mann-Whitney test (two-tailed), ns p > 0.05, * p < 0.05, ** p < 0.01.