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Fig. S5
Timeline of regeneration in 36 Tg(zop:nfsb-EGFP)nt19 37 after MTZ treatment, related to Figure 3.
Tg(zop:nfsb-EGFP)nt19 fish were placed 38 in egg water containing 10mM Metronidazole and treated for 24 hours, then 39 returned to normal egg water to recover. Eyes were removed and proliferation 40 assessed by PCNA staining. Times of recovery observed were pretreatment (A), 0h. 41 recovery (B), 52h. recovery (C), 72h. recovery (D), 96h. recovery (E), and 28 days 42 recovery (F). Scale bar is 100μm.
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