ZFIN Image: Keightley et al., 2017, Fig. S2

Image

Figure Caption

Fig. S2

Depletion of definitive haemopoiesis in mne (supports Fig. 1b)

Whole-mount in situ hybridization showing: a. Erythromyeloid specification marked by gata1, lmo2 and pu.1 expression proceeds in mne;

b. HSC specification marked by runx1 and myb proceeds in mne.

c,d. At 72 and 96 hpf, HSCs are depleted in mne while O-dianisidine staining shows haemoglobinised erythrocyte abundance is unaffected;

e. Decreased numbers of cells expressing mpl and itga2b shows that thrombocytes are depleted in mne. Where head and tail images of the same embryo were spliced to maintain in-focus focal plane, a dashed line indicates the junction; panels a-e scale bar = 200 μm.

Acknowledgments

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