ZFIN Image: Mitchell et al., 2017, Fig. 3

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Figure Caption

Fig. 3

FL-DSLM imaging of 5 dpf NFKB:EGFP zebrafish larvae. The camera exposure time was 1 s with 1 μm slice resolution. Extended orthogonal maximum projections (10 μm) of (A) the intensity and (B) the lifetime (both have a gamma correction of 0.5 applied) highlight the change in lifetime between the GFP muscle and FITC vasculature, despite the only small variation in color. The white lines denote the center of the displayed projections. (C) Absorption (dashed line) and emission (solid line) spectra of EGFP (black) and FITC (gray). (D) Segmented volume outlining the FITC-labelled vasculature, the dorsal aorta (DA) and intersegmental vessels (Se) can be clearly identified. The scale bar is 50 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Opt. Lett.