ZFIN Image: Goldman et al., 2017, Fig. S5

Image

Figure Caption

Fig. S5

Additional CM Regeneration Enhancer Elements (CREEs) (related to Figure 5)

(A) Genome browser snapshots showing enrichment of H3.3 from uninjured (top track, dark blue) and regenerating CMs (bottom track, dark red). Dashed grey box shows the loci with increasing H3.3 used to generate the IN13zgc136858ENfos:EGFP and 22sema3aaENfos:EGFP enhancer reporter lines. Transcription start sites, blue arrows. Gene bodies, blue dashed line. Top right, x-axis.

(B) Three of five CREE transgenics (generated with elements 5anillinEN, 3endothelin2EN, and 103runx1EN) showed induced EGFP 2 days after amputation (2 dpa) of caudal fins. BF, bright field. Scale bar, 200 μm.

Acknowledgments

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Reprinted from Developmental Cell, 40, Goldman, J.A., Kuzu, G., Lee, N., Karasik, J., Gemberling, M., Foglia, M.J., Karra, R., Dickson, A.L., Sun, F., Tolstorukov, M.Y., Poss, K.D., Resolving Heart Regeneration by Replacement Histone Profiling, 392-404.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell