Fig. 6
Constitutively activated Akt protect neuronal cell from heatshock and UV light-induced apoptosis. Representative images of cryosectioned control mRNA (A?C) or myr-Akt mRNA-injected embryos, (D?F) stained using TUNEL (red) and the nuclear dye, Sytox (green). Images on right side of panels are TUNEL only, while left side are overlaid images of TUNEL and Sytox staining. (A,D) Embryos without treatment; (B,E) embryos administered heatshock; (C,F) embryos administered UV light. Scale bar = 50 ?m. (G) Graphical representation of percentages of TUNEL-positive cells in each treatment group. N = 6 images quantified per treatment group (*P < 0.05; #P < 0.001).