|
Fig. 3
Phosphoinositide 3-kinase inhibition associated brain size decrease was recovered by myr-Akt overexpression. (A) Western immunoblots using antibody for phosphorylated Akt on embryos of wide-type and injected with myr-Akt mRNA treated with 8 µM, 16 µM, or without LY294002 or control mRNA (encoding GFP), with total Akt as a loading control. (B) Morphology of 24 hpf embryos of wild type and myr-Akt mRNA-injected group, treated with 8 µM, 16 µM, or without LY294002. GFP mRNA was injected as control mRNA. The first and third panel from the top, scale bar = 500 μm; the second and fourth panel displayed a close-up of the head region, scale bar = 200 μm. (C) Relative brain height of embryos with GFP or myr-Akt injected, treated with 8 µM, 16 µM, or without LY294002. Results are from three independent microinjection experiments, each with 25 embryos per group. P < 0.05 compared with the wide-type control group.