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Fig. 2 S1
ref mutants display craniofacial defects.
(A–F) Lateral (A–C) and ventral (D–F) views of embryos at 6 days post-fertilization (dpf), stained with Alcian blue to show the dorsal and ventral cartilage in the head. Embryos homozygous for ref (B,E; n = 9/9) or transheterozgous for ref and b1059 (C,F; n = 5/6) display similar dorsal cartilage formation defects. Specifically, the dorsal ethmoid plate is diminished or absent in these mutants. Scale bars: 60 μm. (D'–F') Cartoons illustrate the dorsal cartilage structures shown in D–F.
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