ZFIN Image: Bower et al., 2017, Fig. 2

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Figure Caption

Fig. 2

Vegfd contributes to the development of parachordal lymphatic endothelial cells. (A-C) Confocal images at 54 hpf showing presence (arrows) or absence (asterisks) of PL formation in representative Tg(fli1a:egfp) vegfc^hu5055, vegfd^uq9bh mutant embryos. (D-F) Quantification of PL formation in vegfd^uq9bh mutant embryos in vegfc^hu5055 wild-type (D), heterozygous (E) and mutant (F) backgrounds. There were significantly fewer PLs formed in vegfd^uq9bh homozygous mutant (n=42) and heterozygous (n=112) embryos when compared with vegfd^uq9bh wild-type (n=63) embryos in a vegfc^hu5055 heterozygous background (E). Error bars represent mean±s.e.m.; ***P<0.001 from one-way ANOVA from three independent clutches of embryos.

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Acknowledgments

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