|
Fig. 8
The toxicity of compound K (CK) and β-cyclodextrin included in compound K (CD-CK) in enhanced green fluorescent protein (EGFP) expression fused to the mitochondrial localization sequence (MLS) of zebrafish COXVIII (MLS-EGFP) and TUNEL assays of CK- and CD-CK-treated digestive systems in zebrafish larvae. (A) Representative whole zebrafish images. The red dotted line indicates the digestive system. Scale bar = 100 μm. (B) Size of the digestive system analysis. (C) The fluorescence intensity analysis. (D) The white arrow indicates the location of apoptosis in the digestive system. Scale bar = 50 μm. (E) Quantification of TUNEL-positive apoptosis cells. TUNEL, TdT-UTP nick end labeling. ** p < 0.01 compared to the normal (NOR) group. *** p < 0.001. + p < 0.05.