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Fig. 2
Small-Molecule Screening to Enhance Homing of Donor Cells
(A) Serial BLI of non-irradiated and 20-Gy-irradiated recipients receiving 500,000 ubi:luc WKM cells.
(B) Average ± SD radiance of engrafted recipients over time, n = 6 individuals/group.
(C) Serial BLI of recipients transplanted with vehicle- and dmPGE2-treated ubi:luc WKM cells.
(D) BLI quantification of recipients in vehicle- and dmPGE2-treated groups, n = 13 individuals/group, data shown are mean ± SD, ?p < 0.05, ??p < 0.01, from a Student's t test.
(E) Survival of recipients from control and dmPGE2-treated groups is significantly different, n = 13 individuals/group.
(F) Example of the BLI readout from a series of tested compounds. All recipients received 200,000 ubi:luc donor cells co-incubated with 10 ?M of compound prior to HCT. Each bar represents one animal. The dashed line shows the highest BLI in one of three control animals for that experimental group.
(G) Second screen of initial hits with n = 3?5 animals per compound. Data show a dashed line at the control mean ± SD. This example shows compound 04-B11 (ergosterol) that was examined in follow-up studies.