ZFIN Image: Tenente et al., 2017, Fig. 3 S1

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Figure Caption

Fig. 3 S1

Analysis of transplanted ERMS arising in CG1-strain syngeneic recpients.

(A,D) Representative images of transplanted fish. ERMS were created in myf5-GFP/mylpfa-mCherry transgenic, CG1-strain syngeneic zebrafish and imaged following 30 days of engraftment. Genotypes denoted to the left. (B,E) Representative histology of transplanted tumors. (C,F) Quantification of differentiation based on histological review (1-less differentiated and 3-most differentiated). **p<0.01 by Chi-square test. (G–P) Representative examples of sort purity following FACS for cells used in limiting dilution cell transplantation experiments. (G–K) Sort purity following FACS for a representative kRAS^G12D-alone expressing ERMS and (L–P) kRAS^G12D+ mylpfa:myf5 expressing ERMS (data is reproduced in lower left panels of Figure 3D and G). Scale bars equal 2 mm (A,D) and 100 μm (B,E).

Acknowledgments

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