ZFIN Image: Tenente et al., 2017, Fig. 1 S3

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Figure Caption

Fig. 1 S3

Analysis of proliferation and apoptosis in zebrafish primary ERMS.

(A) Representative H and E-stained sections and immunohistochemistry for phospho-H3 (pH3) and cleaved caspase-3 (CC3). (B) Quantification of the total number of pH3-positive cells per 400x imaging field. (n=average of 3 fields/tumor section). (C) Quantification of the total number of CC3-positive cells per 400x imaging field (n=average of 3 fields/tumor). Boxes in B–C denote 50% confidence interval and mean, maximum, and minimum shown. kRAS^G12D[K] (N = 5) and kRAS^G12D; mylpfa:myf5 [K+M] (N = 11). (D) Quantification of total number of EdU+ cells per area (n=average of 3 fields/tumor. N = 3 tumors per genotype). *p<0.05 or **p<0.01 in comparison to each kRAS^G12D-alone expressing ERMS (Student’s t-test). Error bars denote +/- STD. Scale bars equal 100 μm (A). Not significant by Student’s t-test (n.s.).

Acknowledgments

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