IMAGE

Fig. 3

ID
ZDB-IMAGE-170222-43
Source
Figures for Heanue et al., 2016
Image
Figure Caption

Fig. 3

Intestinal motility is impaired in rethu2846/+ and rethu2846/2846 larvae.

(A) Spatiotemporal maps (STMs) generated to quantify 400 second recordings of gut motility in live 7dpf zebrafish larvae. WT (ret+/+) larvae exhibit cyclical retrograde (anal to oral) motility patterns in the intestinal bulb (brackets), and anterograde (oral to anal) peristaltic waves in the intestine starting at the intestinal bulb/intestine junction (white arrows); black arrows denote motility end points. STMs of rethu2846/+ larvae with ENS phenotypes and rethu2846/2846 larvae show alterations in cyclical retrograde motility patterns (brackets) and frequency and distance of anterograde motility waves (white to black arrows). (B) Anterograde contraction characterization. Phenotypic (rethu2846/+P) and non-phenotypic (rethu2846/+NP) larvae identified immunohistochemically. Reduced peristaltic frequency in rethu2846/2846 larvae and rethu2846/+ P relative to WT (one-way ANOVA, p = 0.0004; Bonferroni’s post-hoc, p = 0.0001 and p = 0.0367), and also reduced in rethu2846/2846 larvae as compared to rethu2846/+ NP (p = 0.0002) and rethu2846/+ P (p = 0.0035). Reduced contraction travel distance in rethu2846/2846 larvae relative to WT (one-way ANOVA, p = 0.0005; Bonferroni’s post-hoc, p = 0.0002), and relative to both rethu2846/+ P (p = 0.0232) and rethu2846/+ NP (p = 0.0237). Reduced contraction velocity in rethu2846/2846 relative to WT (one-way ANOVA, p = 0.0212; Bonferroni’s post-hoc, p = 0.0138). Increased contractions interval in rethu2846/2846 larvae relative to WT and rethu2846/+ NP (one-way ANOVA, p = 0.0078; Bonferroni’s post-hoc, p = 0.0053 and p = 0.0097). (C) Loss of anterograde contractions in rethu2846/hu2846 larvae compared to WT and rethu2846/+ NP (#, Fisher’s exact, p = 0.0198 and p = 0.028). rethu2846/2846 larvae show retrograde motility changes relative to all other genotypes, with contractions losing cyclic patterns (Fisher’s exact, vs. WT: p = 0.0001, vs. rethu2846/+ NP: p = 0.001, and vs. rethu2846/+ P: p = 0.0082). (D) Equivalent analysis in the SAGFF234A;UAS:GFP background, expressing GFP in ENS cells and their processes, enables mapping of contraction endpoints relative to cell body position (GFP+HuC/D+ cells, rs = 0.8857, p = 0.0333) and cell process (GFP+HuC/D+ process, rs = 0.942, p = 0.0167). Black lines indicate linear fits.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.