Fig. 2
Clock-controlled aanat2 mRNA rhythm is abolished in the pineal gland of Tg(aanat2:EGFP-ΔCLK) larvae.
(A) Tg(aanat2:EGFP-ΔCLK) larvae (ΔCLK) exhibit arrhythmic pineal aanat2 mRNA levels under DD compared with a robust rhythm in their WT siblings (p<0.0001, two-way ANOVA), indicating that the pineal gland molecular clock is disrupted. Each value represents the mean optical density ± SE of the pineal aanat2 whole-mount ISH signal (n = 10−15 larvae). Different letters represent statistically different values (p<0.05, Tukey's test). (B) Representative whole-mount ISH aanat2 signals (8 dpf, dorsal view) in the pineal glands of WT sibling larvae (upper panel) and Tg(aanat2:EGFP-ΔCLK) larvae (ΔCLK; bottom panel). Black and gray bars represent subjective night and day, respectively. CT, circadian time.