Fig. S3

Fig. S3

Tbx20 and Tbx20-GR overexpression phenotypes. (A and B) Uninjected control embryos (A) have begun to gastrulate by 6 h post fertilization, but overexpression of Tbx20 by injection of tbx20 mRNA disrupts gastrulation (B). (C and D) In the absence of DXS, 23.5% (12 out of 51 embryos from the tbx20^LA1110 heterozygous crosses) of tbx20-GR injected embryos showed a characteristic tbx20^LA1110 cardiac edema phenotype (D), demonstrating a failure to rescue without DXS induction. The remainder of the embryos displayed normal morphology (C). (E and F) Tbx20^LA1110-GR overexpression in the presence of DXS induction did not rescue tbx20^LA1110 mutants or induce developmental abnormalities, as 27.6% (16 out of 58 embryos from the tbx20^LA1110 heterozygous crosses) of the embryos displayed the characteristic tbx20^LA1110 cardiac edema phenotype (F) while the remainder were morphologically normal (E).