ZFIN Image: Lu et al., 2017, Fig. 8

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Figure Caption

Fig. 8

Tbx20's cardiac expansion activity requires its DNA binding and transcriptional activation domains. (A) Schematic diagram of the Tbx20-GR, Tbx20-Δtbx-GR (a T-box-defective form of Tbx20), Tbx20-Δact-GR (a transactivation-defective form of Tbx20), Tbx20-Δrep-GR (a transrepression-defective form of Tbx20), and TbxVP16-GR (a transactivation-only form of Tbx20) fusion proteins. (B–D) Tbx20-GR-injected embryos treated with DXS have significantly more cardiomyocytes (n=6). (E–G) Tbx20-Δtbx-GR overexpression did not significantly enhance cardiomyocyte production (n=6). (H–J) Overexpression of Tbx20-Δact-GR did not significantly enhance cardiomyocyte production (n=6). (K–M) Overexpression of Tbx20-Δrep-GR significantly increased cardiomyocyte number (n=6). (N–P) Overexpression of TbxVP16-GR significantly increased cardiomyocyte production (n=6). Error bars indicate standard deviations. ns indicates there is no significant difference compared with control embryos. Asterisks indicate a significant difference (*p<0.05, **p<0.01, ***p<0.001).

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.