ZFIN Image: Lu et al., 2017, Fig. 7

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Figure Caption

Fig. 7

Cardiomyocyte-specific Tbx20 expression promotes cardiomyocyte production. (A) Schematic representation of the myl7:Tbx20 transgene used for inducible and cardiomyocyte-specific overexpression of Tbx20. The induction of Tbx20 in myl7-expressing cells is controlled by treatment with TBF (tebufenozide). (B–D) Representative confocal projections of myl7:nucGFP hearts from TBF-treated control embryos (B), non-TBF-treated myl7:Tbx20 injected embryos (C) and TBF-treated myl7:Tbx20-injected embryos (D) at 2 dpf. (E) Graph of the average number of nucGFP-positive cardiomyocytes in TBF-treated controls, non-TBF-treated myl7:Tbx20-injected embryos and TBF-treated myl7:Tbx20-injected embryos. Cardiomyocyte-specific overexpression of Tbx20 increased cardiomyocyte production. Error bars indicate standard deviations. ns indicates no significant difference compared with control embryos (n=6, p>0.05). Asterisks indicate a significant increase compared with control embryos (n=6, ***p<0.001).

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Acknowledgments

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Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.