ZFIN Image: Rydeen et al., 2016, Fig. 4

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Figure Caption

Fig. 4

Restoring FGF signaling rescues SHF addition but not ventricular integrity.

(A,B) Graphs indicating fold difference of mRNA relative to β-actin assayed with RT-qPCR of fgf8a expression in whole embryos and isolated hearts at 48 hpf. (C) Graph depicting quantification of ventricular addition to the OFT (control n = 8, CA-Fgfr n = 6, Cyp26 deficient n = 10, Cyp26 deficient+CA-Fgfr n = 6). (D) Graph of ventricular cardiomyocyte counts at 48 and 72 hpf (n = 10 per group). (E–H) Confocal images of optical slices from hearts of Tg(myl7:Kaede) and Tg(myl7:Kaede);Tg(hsp70:ca-fgfr1) embryos at 48 hpf after heat-shock at 24 hpf and photoconversion at 36 hpf. Brackets indicate added ventricular cells (green only). (I–L) IHC for hearts from Tg(myl7:DsRed-NLS) and Tg(myl7:DsRed-NLS);Tg(hsp70:ca-fgfr1) at 48 hpf after heat-shock at 24 hpf. Arrows indicate ectopic cardiomyocytes. Error bars are SEM, asterisk denotes p < 0.05 by Student’s t test. Frontal views with anterior up (E–L); n > 20 embryos per group (E–L). Scale bar: 50 μm.

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Acknowledgments

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