ZFIN Image: Sidhaye et al., 2016, Fig. 4

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Figure Caption

Fig. 4

Enterocytes in gsp/myoVb mutants show presence of microvillus inclusions.

Confocal .microscopy analysis of whole mount (A, B) and cryosections (C, D1, D2) of the anterior gut of wild type sibling (A, C) and gsp/myoVb mutant (B, D1, D2) larvae stained for lyn-EGFP and F-actin (phalloidin) at 6dpf. In all, 3 mutants and 4 siblings were examined in whole mounts whereas 7 mutants and 7 siblings were analysed by cryosectioning. Confocal sections of anterior gut (E) and proximal midgut (F) from the same gsp/myoVb mutant stained for phalloidin. In total 17 mutant animals from three different sets were analysed this way. The graph (G) shows the comparison between the number of inclusions in the anterior versus proximal midgut in mutants. Each line indicates one animal and different colours indicate values from different experimental sets. The arrows indicate phalloidin or lyn-EGFP labelled inclusion bodies in enterocytes. The asterisk represents statistically significant difference (p < 0.0001) by paired t-test. Scale bars: in B and D2 = 20 μm and in E = 10 μm. Abbreviation: sib = sibling.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Mechanisms of Development, 142, Sidhaye, J., Pinto, C.S., Dharap, S., Jacob, T., Bhargava, S., Sonawane, M., The zebrafish goosepimples/myosin Vb mutant exhibits cellular attributes of human microvillus inclusion disease, 62-74, Copyright (2016) with permission from Elsevier. Full text @ Mech. Dev.