ZFIN Image: Qi et al., 2016, Fig. S1 E

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Figure Caption

Fig. S1 E

NgAgo based flt1 gene knockdown caused excessive angiogenesis phenotype in zebrafish. (A) Microscopy analysis of blood vessel in control and NgAgo-2nls and flt1 FW-guide DNA 1 coinjected embryos, lateral view. Green arrowhead indicates intersegmental vessel (ISVs). (B) Relative mRNA levels of zebrafish flt1 in 30 hpf control and flt1 FW-guide DNA 1 and NgAgo-2nls mRNA coinjected embryos. T-test; ****, P<0.0001. (C) Statistical analysis of phenotype efficiency in control and NgAgo-2nls and flt1 FW-guide DNA 1 coinjected embryos. χ² test; ****, P<0.0001. The Tg(kdrl:EGFP) transgenic line was described in our previous work (Wang X, Ling CC, Li L, Qin Y, Qi J, Liu X, et al. MicroRNA-10a/10b represses a novel target gene mib1 to regulate angiogenesis. Cardiovascular research 2016, 110(1): 140-150.).

Acknowledgments

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