ZFIN Image: Qi et al., 2016, Fig. S1 C

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Figure Caption

Fig. S1 C

NgAgo based kdrl gene knockdown caused angiogenic defects phenotype in zebrafish. (A) Microscopy analysis of blood vessel in control and NgAgo-2nls and kdrl FW-guide DNA 1 coinjected embryos, lateral view. Green arrowhead indicates intersegmental vessel (ISVs). (B) Relative mRNA levels of zebrafish kdrlin 30 hpf control and kdrl FW-guide DNA 1 and NgAgo-2nls mRNA coinjected embryos. T-test; ****, P<0.0001. (C) Statistical analysis of phenotype efficiency in control and NgAgo-2nls and kdrl FW-guide DNA 1 coinjected embryos. χ² test; ****, P<0.0001. The Tg(kdrl:mCherry) transgenic line was described in our previous work (Jiang Q, Lagos-Quintana M, Liu D, Shi Y, Helker C, Herzog W, et al. miR-30a regulates endothelial tip cell formation and arteriolar branching. Hypertension 2013, 62(3): 592-598.).

Acknowledgments

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