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Fig. 2 S1
Microglia, macrophages, pericytes, and/or neutrophils do not modulate trunk vascular patterning.
(A–C) 154 hpf TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR);Tg(kdrl:EGFP) irf8+/+ (A), irf8+/- (B), and irf8-/- (C) trunk vasculature visualized by Tg(kdrl:EGFP) expression. Animals were treated with 2 mM Mtz between 30 and 54 hpf. Ectopic vessels after radial glial ablation emerge similarly in irf8+/+, irf8+/-, and irf8-/- fish. Scale bar, 100 µm. (D) Quantification of average number of somites that showed ectopic blood vessels in irf8+/+, irf8+/-, and irf8-/- larvae (10 somites examined per animal; ≥12 animals examined per genotype). (E–G) 154 hpf TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR);Tg(kdrl:EGFP) pdgfrb+/+ (E), pdgfrb+/- (F), and pdgfrb-/- (G) trunk vasculature visualized by Tg(kdrl:EGFP) expression. Animals were treated with 2 mM Mtz between 30 and 54 hpf. Ectopic vessels after radial glial ablation emerge similarly in pdgfrb+/+, pdgfrb+/-, and pdgfrb-/- fish. Scale bar, 100 µm. (H) Quantification of average number of somites that showed ectopic blood vessels in pdgfrb+/+, pdgfrb+/-, and pdgfrb-/- larvae (10 somites examined per animal; ≥11 animals examined per genotype). (I and J) 154 hpf TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR);Tg(kdrl:EGFP) (I) and TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR);Tg(kdrl:EGFP);Tg(mpx:gal4) (J) trunk vasculature visualized by Tg(kdrl:EGFP) expression. Animals were treated with 2 mM Mtz between 30 hpf and 154 hpf. Ectopic vessels after radial glial ablation emerge similarly in fish with or without the mpx:gal4 transgene. Scale bar, 100 µm. (K) Quantification of average number of somites that showed ectopic blood vessels in fish with or without the mpx:gal4 transgene (10 somites examined per animal; ≥17 animals examined per condition). In all panels, values represent means ± SEM.