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Fig. 5
Myosin accumulates in basal foci during optic cup morphogenesis.
(A?D) Live-imaging analysis of tg(actb1:myl12.1-eGFP) embryos reveals myosin accumulation at the apical lens (purple arrowheads) and basal retina (orange arrowheads) between 19 and 20.5 hpf. Antero-posterior (a?p) and medio-lateral (m-l) axes are indicated. (E?H) Myosin accumulates in transient foci (orange arrows) at the basal cortex. (I?P) Time-lapse analysis of myosin foci at the basal surface plane in embryos injected with lyn-tdTomato RNA reveals that the protein accumulates at the peripheral cortex in scattered cells. (Q) The box plot shows a significant difference in foci stability between control and blebbistatin (150 µM) treated embryos (T-test, n = 21). fb = forebrain; nr = neural retina; lv = lens vesicle. Scale bars = 50 µm in A?D, 20 µm in E?H, and 10 µm in I?P.