Fig. 3

Fig. 3

Microglia interact differently with U251, U87, and HT1080 cells. (A) Confocal time series showing microglia (green) responding to U251 cells (red) in the zebrafish optic tectum (recording times indicated; see also Supplementary Movie S1). (A₃–A₆) Time series highlighting a microglial cell closely interacting with a U251 cell for more than 60 min. White arrowhead marks the microglial cell interacting with the U251 cell. (A₇–A₁₀) Time series highlighting a microglial cell constantly sending and retracting processes toward U251 cells. White asterisk marks a microglial cell sending and retracting processes. White arrows mark microglial processes toward U251 cells. Scale bars for (A–A₂): 30 μm. Scale bars for (A₃–A₁₀): 15 μm. (B) Confocal time series showing microglia (green) responding to U87 cells (red) in the zebrafish optic tectum (recording times indicated; see also Supplementary Movie S2). (B₃–B₆) Time series highlighting a microglial cell closely interacting with U87 cells. White asterisk marks a microglial cell sending and retracting processes. White arrows mark microglial processes toward U87 cells. Scale bars for (B–B₂): 30 μm. Scale bars for (B₃–B₆): 20 μm. (C) Confocal time series showing microglia (green) responding to HT1080 cells (red) in the zebrafish optic tectum (recording times indicated; see also Supplementary Movie S3). (C₃–C₆) Time series highlighting microglia engulfing and phagocytosing HT1080 cells. Arrowheads indicate microglia engulfing and phagocytosing HT1080 cells. Scale bars for (C–C₆): 30 μm. All images represent maximum intensity projections of confocal stacks. Images were captured using an Andor spinning disk confocal microscope with a 20 × /NA 0.75 objective.