IMAGE

Fig. 2

ID
ZDB-IMAGE-161206-41
Source
Figures for Carneiro et al., 2016
Image
Figure Caption

Fig. 2

Telomeres shorten in WT gut and muscle, but not in testis, with aging reaching the length of tert-/- tissues.

A-C) Representative images of telomere restriction fragment (TRF) analysis of genomic DNA by Southern Blot (random primer-labelled telomeric probe (TTAGGG)n 32P-dCTP) and quantifications of median telomere length. Black and red stars represent WT and tert-/- larvae telomeres, respectively, in quantifications of median TRF over time. WT telomeres shorten linearly with aging from 3 to 24 months, in A) gut (N = 3–4 per time point) and B) muscle (N = 3–4 per time point), stabilizing in later ages. C) No significant telomere shortening is detected in the testis (N = 5–6 per time point). Around 20 months of age WT telomeres reach the shorter length of 12 month-old tert-/- in the gut (graph in fig A–ca. 6.8 Kb) and muscle (graph in fig B–ca. 8.5 Kb) but not in testis (graph in fig C), indicated by blue arrow. N = 3–4 per time point for tert-/- gut and muscle, N = 4–6 per time point for tert-/- testis. TRF mean sizes were calculated as previously described [54]. Data are represented as mean +/- SEM.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.