ZFIN Image: Widziolek et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Gingipains are crucial virulence factors of Pg in the zebrafish larvae infection model.

Kaplan-Meier survival plots of zebrafish larvae infected with Pg W83 (WT), ?RgpA deficient mutant, ?RgpB deficient mutant, ?Kgp deficient mutant, Kgp and RgpA deficient mutant (?K/Ra) or gingipain null mutant (?K/Ra-b) (a). Percentage of dead, oedematous or healthy WT Pg W83 or gingipain mutant-infected larvae after 24 (b) and 72?hpi (c) respectively. Morphology of zebrafish larvae 72?hpi with WT or ?K/Ra-b mutant of Pg W83 (d). PBS was injected as a control. All experiments were performed at least 3 times. Bars represent means?�?SD. In all experiments WT Pg or its gingipain mutants were injected into 30?hpf zebrafish larvae at 5?�?10^4?CFU. Black arrow indicates oedemas in WT Pg W83-infected larvae. Scale bar?=?500??m. Comparisons between survival curves were made using the log rank test. Differences between groups displaying oedema were measured using One-way ANOVA after normality was assured.

Figure Data

Phenotype details

Acknowledgments

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