Fig. 2
phox2bb:EGFP exhibits a high degree of colocalization with phox2bb mRNA. A and C show confocal images of cross-sections through the trunk. At 54 (A) and 60 (C) hpf, phox2bb mRNA (red) largely colocalizes with phox2bb:EGFP (green). Insets show enlargements of outlined cells, overlay, phox2bb:EGFP, phox2bb mRNA (from left to right). At 54 (B) and 60 (D) hpf, quantification in percent of phox2bb:EGFP and phox2bb mRNA colocalization in the three regions analyzed. Note that the dotted line between the measured values does not show the continuous percentage of coexpression between the anterior, mid and posterior values, but is drawn to facilitate visualization of coexpression trends. ie, intestinal epithelium. Scale bar = 10 μm.