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Fig. S1

ID
ZDB-IMAGE-161103-13
Source
Figures for Sicca et al., 2016
Image
Figure Caption

Fig. S1

WT and mutated Kir4.1 expression and distribution in U251 astrocytoma cells. (a) Immunofluorescence stainings of U251 astrocytoma cells with anti-Kir4.1 pAb (red) and FITC-conjugated phallacidin (green) to stain actin filaments show that the endogenous Kir4.1 channels are mainly distributed in cytoplasmic perinuclear area and scarcely at plasma membrane. Scale bars: 10 µm. (b) RT-PCR analysis using primers to detect specifically recombinant Kir4.1 mRNA expression (Forward: Xpress epitope/pcDNA 3.1/His, Life technologies; Kir4.1 Reverse: TCAGACATTGCTGATGCGCAC) in stably infected U251 cells reveals no differences between WT (1) and R18Q Kir4.1 (2) expressing cells. GAPDH housekeeping gene normalizes the amount of template used.

Acknowledgments
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