ZFIN Image: Dranow et al., 2016, Fig. 5

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Figure Caption

Fig. 5

(A) The maximum sized oocytes observed in 55 dpf bmp15^+/- ovaries are mid stage II (~280 µm in diameter), while the maximum sized oocytes observed in bmp15^-/- sibling ovaries are early stage II, (~150 µm in diameter; E). (B) Representative magnified view of a mid stage II oocyte follicle in bmp15^+/- ovary showing cyp19a1a:EGFP -expressing granulosa cells (arrows) and a developed vitelline envelope (between dotted lines). (C) Representative early stage II oocyte found in 55 dpf bmp15^+/- ovaries and magnified view in (D). (F) Representative magnified view of an early stage II oocyte follicle in TgBAC(cyp19a1a:EGFP);bmp15^-/- ovaries showing that while GFP is expressed in theca cells (green), it is not expressed in any granulosa cells, as is the case for similarly staged bmp15^+/- oocytes (C, D). In all images, cyp19a1a:EGFP is green and DNA is blue. IB, stage IB oocyte; II, stage II oocyte; Oo, oocyte; ve, vitelline envelope. Arrows indicate granulosa cell nucleus. Scale bars: 100 µm (A, C, E), 50 µm (B, D, F).

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Acknowledgments

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