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Fig. 2
The effect of novel thalidomide analogs on vessel length. fli1:EGFP zebrafish were incubated with vehicle or compounds for 24 hours, at which point vessel outgrowth and number were measured. A. Images of zebrafish exposed to the vehicle or test compounds. Zebrafish with the vehicle (0.1% DMSO) show normal patterning of the ISVs, zebrafish exposed to CPS49 show reduction in vessel outgrowth (white arrow), loss of vascular connectivity and a decrease in the number of forming blood vessels. Treatment with C4 (1.5 µg/mL), C18 (50 µg/mL), C81 (50 µg/mL), C82 (5 µg/mL), C83 (20 µg/mL) and C84 (50 µg/mL) show reduction in vessel out growth (white arrow) and in some cases loss of vessel (white asterisks). B. Stalk length & C. stalk number are reduced. Compounds are shown at the lowest concentration producing an effect. The data represent the mean + the standard error of the mean.