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Fig. 3
arl13b-/- mutants have shorter photoreceptor outer segments and cilia at 4 dpf. (A, B) Immunohistochemistry on transverse cryosections from 4 dpf wild-type and arl13b-/- mutant retinas stained for rhodopsin (red). (C) Quantification of the length of rhodopsin staining along the long axis of the photoreceptor. (D, E) Immunohistochemistry for acetylated α-tubulin (red) and Ift88 (green). (F) Quantification of average cilia length. All sections were counterstained with DAPI to label nuclei. Scale bars: 10 µm. Error bars: ± SEM. *P < 0.0001 using Student′s t-test with Welch′s correction.
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Full text @ Invest. Ophthalmol. Vis. Sci.