ZFIN Image: Ji et al., 2016, Fig. 2

Image

Figure Caption

Fig. 2

Functional analysis of mutant pitx2HD transcripts. (A) RNA in situ hybridizations revealed normal pitx2 mRNA expression in wild-type, pitx2HD^-/^- and pitx2c^-^/- embryos, including asymmetric pitx2c in lateral plate mesoderm (arrow) at the 18 somite stage (18 ss). L=left; R=right. (B) Microinjection of wild-type pitx2c mRNA into wild-type embryos resulted in severe developmental defects or lethality at 2 dpf, which was not frequently observed in controls injected with gfp mRNA. Most embryos injected with pitx2c mRNA containing the HD^sny15 mutation developed normally. n=number of embryos analyzed (pooled from three experiments).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 416(1), Ji, Y., Buel, S.M., Amack, J.D., Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs, 69-81, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.